Detection of specific hybridoma clones by replica immunoadsorption of their secreted antibodies.

A sensitive and rapid method for the detection of monoclonal antibodies secreted by hybridomas is described. Mouse myeloma cells are fused with spleen cells from immunized mice and directly cloned in soft agarose containing selective medium; hybrid clones can be seen after a week. Nitro-cellulose fi...

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Bibliographic Details
Main Authors: Sharon, J, Morrison, S L, Kabat, E A
Format: Artigo
Language:Inglês
Published: 1979
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC383263/
https://ncbi.nlm.nih.gov/pubmed/286325
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