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Generation of T Cell Receptor Retrogenic Mice: Improved Retroviral Mediated Stem Cell Gene Transfer

The utilization of retrogenic mice offers a rapid and flexible approach to T-cell receptor transgenic mice. By transducing bone marrow progenitor cells with retrovirus that encodes a given TCR alpha and beta subunit, TCR retrogenic mice can be generated in as little as 4-6 weeks whereas conventional...

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Detalhes bibliográficos
Main Authors: Bettini, Matthew L., Bettini, Maria, Nakayama, Maki, Guy, Clifford S., Vignali, Dario A.A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3832243/
https://ncbi.nlm.nih.gov/pubmed/24008379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.111
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