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Three-dimensional electron crystallography of protein microcrystals

We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). Lysozyme microcrystals were frozen on an electron microscopy grid, and electron diffraction data collected to 1.7 Å re...

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Bibliografiske detaljer
Main Authors: Shi, Dan, Nannenga, Brent L, Iadanza, Matthew G, Gonen, Tamir
Format: Artigo
Sprog:Inglês
Udgivet: eLife Sciences Publications, Ltd 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3831942/
https://ncbi.nlm.nih.gov/pubmed/24252878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.01345
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