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Engineered solubility tag for solution NMR of proteins
The low solubility of many proteins hinders large scale expression and purification as well as biophysical measurements. Here, we devised a general strategy to solubilize a protein by conjugating it at a solvent-exposed position to a 6 kDa protein that was re-engineered to be highly soluble. We appl...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Blackwell Publishing Ltd
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3831679/ https://ncbi.nlm.nih.gov/pubmed/23963792 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2337 |
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