Transfection of human lymphoblastoid cells with herpes simplex viral DNA.

The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells for...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Miller, G, Wertheim, P, Wilson, G, Robinson, J, Geelen, J L, van der Noordaa, J, van der Eb, A J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1979
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC383102/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/218224/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.2.949
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