A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy

We demonstrate a cytotoxicity evaluation of tissue adhesive using Raman spectroscopy. This method allows for quantitative, label-free, non-invasive and rapid monitoring of the biochemical changes of cells following tissue adhesive treatment. Here, we show the biochemical property changes in mouse fi...

Cur síos iomlán

Na minha lista:
Sonraí Bibleagrafaíochta
Main Authors: Jung, Gyeong Bok, Lee, Young Ju, Lee, Gihyun, Park, Hun-Kuk
Formáid: Artigo
Teanga:Inglês
Foilsithe: Optical Society of America 2013
Ábhair:
Spectroscopic Diagnostics
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3829560/
https://ncbi.nlm.nih.gov/pubmed/24298425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.4.002673
Clibeanna: Cuir Clib Leis
Gan Chlibeanna, Bí ar an gcéad duine leis an taifead seo a chlibeáil!
id pubmed-3829560
record_format dspace
spelling pubmed-38295602013-12-02 A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy Jung, Gyeong Bok Lee, Young Ju Lee, Gihyun Park, Hun-Kuk Biomed Opt Express Spectroscopic Diagnostics We demonstrate a cytotoxicity evaluation of tissue adhesive using Raman spectroscopy. This method allows for quantitative, label-free, non-invasive and rapid monitoring of the biochemical changes of cells following tissue adhesive treatment. Here, we show the biochemical property changes in mouse fibroblast L929 cells and cellular DNA following tissue adhesive (Dermabond) treatment using Raman spectroscopy. The Raman band intensities were significantly decreased when the cells were treated with Dermabond as compared to control cells. These results suggest denaturation and conformational changes in proteins and degradation of DNA related to cell death. To support these conclusions, conventional cytotoxicity assays such as WST, LIVE/DEAD, and TUNEL were carried out, and the results were in agreement with the Raman results. Thus, Raman spectroscopy analysis not only distinguishes between viable and damaged cells, but can also be used for identification and quantification of a cytotoxicity of tissue adhesive, which based on the cellular biochemical and structural changes at a molecular level. Therefore, we suggest that this method could be used for cytotoxic evaluation of tissue adhesives by rapid and sensitive detection of cellular changes. Optical Society of America 2013-10-29 /pmc/articles/PMC3829560/ /pubmed/24298425 http://dx.doi.org/10.1364/BOE.4.002673 Text en © 2013 Optical Society of America
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Spectroscopic Diagnostics
spellingShingle Spectroscopic Diagnostics
Jung, Gyeong Bok
Lee, Young Ju
Lee, Gihyun
Park, Hun-Kuk
A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy
description We demonstrate a cytotoxicity evaluation of tissue adhesive using Raman spectroscopy. This method allows for quantitative, label-free, non-invasive and rapid monitoring of the biochemical changes of cells following tissue adhesive treatment. Here, we show the biochemical property changes in mouse fibroblast L929 cells and cellular DNA following tissue adhesive (Dermabond) treatment using Raman spectroscopy. The Raman band intensities were significantly decreased when the cells were treated with Dermabond as compared to control cells. These results suggest denaturation and conformational changes in proteins and degradation of DNA related to cell death. To support these conclusions, conventional cytotoxicity assays such as WST, LIVE/DEAD, and TUNEL were carried out, and the results were in agreement with the Raman results. Thus, Raman spectroscopy analysis not only distinguishes between viable and damaged cells, but can also be used for identification and quantification of a cytotoxicity of tissue adhesive, which based on the cellular biochemical and structural changes at a molecular level. Therefore, we suggest that this method could be used for cytotoxic evaluation of tissue adhesives by rapid and sensitive detection of cellular changes.
author Jung, Gyeong Bok
Lee, Young Ju
Lee, Gihyun
Park, Hun-Kuk
author_facet Jung, Gyeong Bok
Lee, Young Ju
Lee, Gihyun
Park, Hun-Kuk
author_sort Jung, Gyeong Bok
title A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy
title_short A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy
title_full A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy
title_fullStr A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy
title_full_unstemmed A simple and rapid detection of tissue adhesive-induced biochemical changes in cells and DNA using Raman spectroscopy
title_sort simple and rapid detection of tissue adhesive-induced biochemical changes in cells and dna using raman spectroscopy
publisher Optical Society of America
publishDate 2013
url https://ncbi.nlm.nih.gov/pmc/articles/PMC3829560/
https://ncbi.nlm.nih.gov/pubmed/24298425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.4.002673
_version_ 1800103541102084096

Míreanna Comhchosúla