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Self-assembly of microtubules in extracts of cultured HeLa cells and the identification of HeLa microtubule-associated proteins.
Microtubule protein from HeLa cell extracts was purified by multiple cycles of polymerization and depolymerization in the absence of glycerol or other exogenous polymerization-stimulatory agents. Approximately 4-5% of the extract protein was tubulin, of which more than one-half was competent to part...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1979
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC382925/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/284344/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.1.293 |
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