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RNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor.

We have analyzed the RNA produced in vitro by incubating nuclei from HeLa cells infected with adenovirus serotype 2. Our results show that adenovirus-specific RNA is produced at a linear rate for up to 2.5 hr. Hybridization analysis of RNA produced in nuclei isolated 18 hr after infection indicated...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Manley, J L, Sharp, P A, Gefter, M L
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1979
Schlagworte:
Research Article
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC382896/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/284328/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.76.1.160
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