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Direct oligonucleotide synthesis onto super-paramagnetic beads
Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedu...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3828674/ https://ncbi.nlm.nih.gov/pubmed/23942380 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbiotec.2013.08.006 |
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