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High-throughput screening and directed evolution of terpene synthase-catalyzed cylization()
[Image: see text] A synthetic substrate enables a new colorimetric screen for terpene synthase cyclization activity, facilitating the engineering of these enzymes. Using directed evolution, the thermostability of the sesquiterpene synthase BcBOT2 was increased without the loss of other properties. T...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2013
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3828198/ https://ncbi.nlm.nih.gov/pubmed/23532864 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201301362 |
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