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Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfolded proteins using chemostat cultures

Pichia pastoris is widely used as a host system for heterologous protein expression in both academia and industry. Production is typically accomplished by a fed-batch induction process that is known to have negative impacts on cell physiology that impose limits on both protein yields and quality. We...

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Autors principals: Hesketh, Andrew R., Castrillo, Juan I., Sawyer, Trevor, Archer, David B., Oliver, Stephen G.
Format: Artigo
Idioma:Inglês
Publicat: Springer Berlin Heidelberg 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3825213/
https://ncbi.nlm.nih.gov/pubmed/24022610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-013-5186-1
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