A Fluorescence Method to Detect and Quantitate Sterol Esterification by Lecithin: Cholesterol Acyltransferase

We describe a simple but sensitive fluorescence method to accurately detect the esterification activity of lecithin:cholesterol acyltransferase (LCAT). The new assay protocol employs a convenient mix, incubate and measure scheme. This is possible by using the fluorescent sterol, dehydroergosterol (D...

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Hlavní autoři: Homan, Reynold, Esmaeil, Nadia, Mendelsohn, Laurel, Kato, Gregory J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2013
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3822574/
https://ncbi.nlm.nih.gov/pubmed/23851343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.06.018
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