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Optimization of Large Gel 2D Electrophoresis for Proteomic Studies of Skeletal Muscle
We describe improved methods for large format, 2-dimensional gel electrophoresis (2-DE) that improve protein solubility and recovery, minimize proteolysis, and reduce the loss of resolution due to contaminants and manipulations of the gels, and thus enhance quantitative analysis of protein spots. Ke...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3822429/ https://ncbi.nlm.nih.gov/pubmed/22589104 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201100642 |
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