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Non-invasive monitoring of cell metabolism and lipid production in 3D engineered human adipose tissues using label-free multiphoton microscopy

Non-linear optical microscopy methods can characterize over time multiple functional properties of engineered tissues during development. Here, we demonstrate how the combined use of third-harmonic generation (THG) and two-photon excited fluorescence (2PEF) imaging can provide direct quantitative bi...

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Détails bibliographiques
Auteurs principaux: Chang, Tyler, Zimmerley, Maxwell, Quinn, Kyle P., Lamarre-Jouenne, Isabelle, Kaplan, David L., Beaurepaire, Emmanuel, Georgakoudi, Irene
Format: Artigo
Langue:Inglês
Publié: 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3821986/
https://ncbi.nlm.nih.gov/pubmed/23932290
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.biomaterials.2013.07.066
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