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Development of reverse-transcription PCR techniques to analyse the density and sex ratio of gametocytes in genetically diverse Plasmodium chabaudi infections

We have developed cross-genotype and genotype-specific quantitative reverse-transcription PCR (qRT-PCR) assays to detect and quantify the number of parasites, transmission stages (gametocytes) and male gametocytes in blood stage Plasmodium chabaudi infections. Our cross-genotype assays are reliable,...

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Detalhes bibliográficos
Main Authors: Drew, Damien R., Reece, Sarah E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3818572/
https://ncbi.nlm.nih.gov/pubmed/17889948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molbiopara.2007.08.004
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