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Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI)
Here we present a protocol for generating transgenic embryos in Xenopus laevis and Xenopus tropicalis. The method includes three steps: (1) The preparation of high-speed egg extracts, which facilitates the replacement of protamines in sperm nuclei with nucleosomes and decondenses the chromatin of sp...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3818119/ https://ncbi.nlm.nih.gov/pubmed/22956089 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-992-1_11 |
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