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Separation and characterization of nitrated variants of the major birch pollen allergen by CZE-ESI-μTOF MS
A CZE-ESI-TOF MS method has been optimized for the separation and identification of nitrated variants of the major birch pollen allergen from Betula verrucosa, isoform 1a (Bet v 1a). In-house nitration of recombinant Bet v 1a was done by peroxynitrite. As a BGE, 10 mmol/L ammonium bicarbonate with p...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Blackwell Publishing Ltd
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3816331/ https://ncbi.nlm.nih.gov/pubmed/23857337 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201300151 |
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