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Studying Interactions of Staphylococcus aureus with Neutrophils by Flow Cytometry and Time Lapse Microscopy

We present methods to study the effect of phenol soluble modulins (PSMs) and other toxins produced and secreted by Staphylococcus aureus on neutrophils. To study the effects of the PSMs on neutrophils we isolate fresh neutrophils using density gradient centrifugation. These neutrophils are loaded wi...

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Hlavní autoři: Surewaard, Bas G.J., van Strijp, Jos A.G., Nijland, Reindert
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3814803/
https://ncbi.nlm.nih.gov/pubmed/23893048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50788
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