Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics

NADPH-dependent acetoacetyl-coenzyme A (acetoacetyl-CoA) reductase (PhaB) is a key enzyme in the synthesis of poly(3-hydroxybutyrate) [P(3HB)], along with β-ketothiolase (PhaA) and polyhydroxyalkanoate synthase (PhaC). In this study, PhaB from Ralstonia eutropha was engineered by means of directed e...

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Detalhes bibliográficos
Main Authors: Matsumoto, Ken'ichiro, Tanaka, Yoshikazu, Watanabe, Tsuyoshi, Motohashi, Ren, Ikeda, Koji, Tobitani, Kota, Yao, Min, Tanaka, Isao, Taguchi, Seiichi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2013
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3811355/
https://ncbi.nlm.nih.gov/pubmed/23913421
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01768-13
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