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Asynchronous basepair openings in transcription initiation: CRP enhances the rate-limiting step
The mechanism of isomerization (basepair openings) during transcription initiation by RNA polymerase at the galP1 promoter of Escherichia coli was investigated by 2-aminopurine (2,AP) fluorescence. The fluorescence of 2,AP is quenched in DNA duplex and enhanced when the basepair is distorted or defo...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Nature Publishing Group
2004
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC381006/ https://ncbi.nlm.nih.gov/pubmed/14963488 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7600098 |
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