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Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli
High-level expression of many recombinant proteins in Escherichia coli leads to the formation of highly aggregated protein commonly referred to as inclusion bodies. Inclusion bodies are normally formed in the cytoplasm; however, if a secretion vector is used, they can form in the periplasmic space....
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| Главные авторы: | , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3809847/ https://ncbi.nlm.nih.gov/pubmed/23151747 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps0603s70 |
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