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Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia coli

High-level expression of many recombinant proteins in Escherichia coli leads to the formation of highly aggregated protein commonly referred to as inclusion bodies. Inclusion bodies are normally formed in the cytoplasm; however, if a secretion vector is used, they can form in the periplasmic space....

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Palmer, Ira, Wingfield, Paul T.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2012
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3809847/
https://ncbi.nlm.nih.gov/pubmed/23151747
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps0603s70
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