Nasal PMN response to repeated challenge with endotoxin in healthy volunteers
RATIONALE: We have employed nasal challenge with lipopolysaccharide (LPS) followed by nasal lavage (NL) to experimentally induce and examine upper airway inflammation in human volunteers. It is unclear however whether adaptation within individuals occurs following repeated nasal challenge. This was...
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3808958/ https://ncbi.nlm.nih.gov/pubmed/21391782 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3109/08958378.2011.553247 |
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pubmed-38089582013-10-28 Nasal PMN response to repeated challenge with endotoxin in healthy volunteers Doreswamy, Vinod Alexis, Neil E. Zhou, Haibo Peden, David B. Inhal Toxicol Article RATIONALE: We have employed nasal challenge with lipopolysaccharide (LPS) followed by nasal lavage (NL) to experimentally induce and examine upper airway inflammation in human volunteers. It is unclear however whether adaptation within individuals occurs following repeated nasal challenge. This was a pilot study to determine if repeated nasal LPS challenge yields attenuation of markers of inflammation (primarily neutrophil response) in the NL fluid of healthy humans. METHODS: We employed a 3-day nasal LPS challenge protocol with NL using a “split nose” design. The control and LPS nares received two consecutive day saline (0.9% saline/day) and LPS (2 μg LPS/day) challenges, respectively followed by an LPS (2 μg/day) challenge to each nare on Day 3. NL was performed immediately pre Day 1 challenges and 6-h post nasal LPS challenges on both Days 1 and 3. Markers of inflammation (PMNs/mg, cytokines) were assessed in NL and the inflammatory response to LPS (measured as the difference between pre and post challenge) was evaluated in both nares on Day 3 and compared to Day 1. RESULTS: Significant (p < 0.05) blunting of the LPS-induced polymorphonuclear leukocyte (PMN) response was observed in the nare that received repeated LPS challenges as compared to the control nare (67.60 ± 22.39 vs. 157.8 ± 76.04 PMN/mg) and initial LPS challenge on Day 1 (121 ± 32 PMN/mg). Decreased soluble CD14 and significantly decreased interleukin-8 were also found in the repeat LPS-treated nare. In the LPS-treated nare, the blunted PMN response on Day 3 correlated well with the observed PMN response on Day1 (r = 0.58, p = 0.02). CONCLUSIONS: We show attenuation of PMN response to repeated LPS in the nasal airways in healthy humans. Effect of repeat endotoxin exposure prior to allergen delivery on local airway inflammation in both healthy and atopic subjects can be studied. 2011-02 /pmc/articles/PMC3808958/ /pubmed/21391782 http://dx.doi.org/10.3109/08958378.2011.553247 Text en Copyright © 2011 Informa Healthcare USA, Inc. |
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Article Doreswamy, Vinod Alexis, Neil E. Zhou, Haibo Peden, David B. Nasal PMN response to repeated challenge with endotoxin in healthy volunteers |
description |
RATIONALE: We have employed nasal challenge with lipopolysaccharide (LPS) followed by nasal lavage (NL) to experimentally induce and examine upper airway inflammation in human volunteers. It is unclear however whether adaptation within individuals occurs following repeated nasal challenge. This was a pilot study to determine if repeated nasal LPS challenge yields attenuation of markers of inflammation (primarily neutrophil response) in the NL fluid of healthy humans. METHODS: We employed a 3-day nasal LPS challenge protocol with NL using a “split nose” design. The control and LPS nares received two consecutive day saline (0.9% saline/day) and LPS (2 μg LPS/day) challenges, respectively followed by an LPS (2 μg/day) challenge to each nare on Day 3. NL was performed immediately pre Day 1 challenges and 6-h post nasal LPS challenges on both Days 1 and 3. Markers of inflammation (PMNs/mg, cytokines) were assessed in NL and the inflammatory response to LPS (measured as the difference between pre and post challenge) was evaluated in both nares on Day 3 and compared to Day 1. RESULTS: Significant (p < 0.05) blunting of the LPS-induced polymorphonuclear leukocyte (PMN) response was observed in the nare that received repeated LPS challenges as compared to the control nare (67.60 ± 22.39 vs. 157.8 ± 76.04 PMN/mg) and initial LPS challenge on Day 1 (121 ± 32 PMN/mg). Decreased soluble CD14 and significantly decreased interleukin-8 were also found in the repeat LPS-treated nare. In the LPS-treated nare, the blunted PMN response on Day 3 correlated well with the observed PMN response on Day1 (r = 0.58, p = 0.02). CONCLUSIONS: We show attenuation of PMN response to repeated LPS in the nasal airways in healthy humans. Effect of repeat endotoxin exposure prior to allergen delivery on local airway inflammation in both healthy and atopic subjects can be studied. |
author |
Doreswamy, Vinod Alexis, Neil E. Zhou, Haibo Peden, David B. |
author_facet |
Doreswamy, Vinod Alexis, Neil E. Zhou, Haibo Peden, David B. |
author_sort |
Doreswamy, Vinod |
title |
Nasal PMN response to repeated challenge with endotoxin in healthy volunteers |
title_short |
Nasal PMN response to repeated challenge with endotoxin in healthy volunteers |
title_full |
Nasal PMN response to repeated challenge with endotoxin in healthy volunteers |
title_fullStr |
Nasal PMN response to repeated challenge with endotoxin in healthy volunteers |
title_full_unstemmed |
Nasal PMN response to repeated challenge with endotoxin in healthy volunteers |
title_sort |
nasal pmn response to repeated challenge with endotoxin in healthy volunteers |
publishDate |
2011 |
url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC3808958/ https://ncbi.nlm.nih.gov/pubmed/21391782 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3109/08958378.2011.553247 |
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