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Extraction of Equine Infectious Anemia Immunodiffusion Antigen with the Aid of the Chaotropic Agent, Thiocyanate

Immunodiffusion antigen from spleens of horses infected with equine infectious anemia virus was prepared by methods employing freeze-thaw cycles and thiocyanate treatment. Thiocyanate (0.5 M) permitted the recovery of the greatest amount of antigen. Furthermore, it was most effective for recovery of...

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Détails bibliographiques
Publié dans:Appl Microbiol
Auteurs principaux: Hart, L. T., Broussard, E. A.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1973
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC380769/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4348466/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.25.2.190-194.1973
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