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Large-Scale Production of Lambda Bacteriophage and Purified Lambda Deoxyribonucleic Acid
Large amounts of a heat-inducible phage lambda mutant (λCI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purifie...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1972
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC380437/ https://ncbi.nlm.nih.gov/pubmed/4553145 |
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