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Preparation of Poliovirus Labeled with Phosphorus-33

Phosphorus-33 ((33)P), a weak (0.25 Mev) beta-emitting isotope of phosphorus with a half-life of 25 days, has been used to label poliovirus in cell culture. HeLa cell monolayers were depleted of phosphate and then labeled by incubating at 37 C in a medium (LM) containing about 10 μCi of (33)P as ort...

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Bibliographic Details
Main Authors: Sobsey, Mark D., Lavigne, Michael E., Cooper, Robert C.
Format: Artigo
Language:Inglês
Published: 1972
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC380397/
https://ncbi.nlm.nih.gov/pubmed/4336662
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