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In vitro evolution of α-hemolysin using a liposome display

In vitro methods have enabled the rapid and efficient evolution of proteins and successful generation of novel and highly functional proteins. However, the available methods consider only globular proteins (e.g., antibodies, enzymes) and not membrane proteins despite the biological and pharmaceutica...

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Main Authors: Fujii, Satoshi, Matsuura, Tomoaki, Sunami, Takeshi, Kazuta, Yasuaki, Yomo, Tetsuya
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3801045/
https://ncbi.nlm.nih.gov/pubmed/24082135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1314585110
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