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Simplified Method for Purification of Clostridium perfringens Type A Enterotoxin

Purification of Clostridium perfringens type A enterotoxin from sporulated cells was simplified. The method consisted of precipitation of the enterotoxin from the extract of sonically treated cells at 40% saturation of ammonium sulfate at pH 7, differential solubilization in 0.02 M phosphate buffer,...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Appl Microbiol
Hauptverfasser: Sakaguchi, Genji, Uemura, Takashi, Riemann, Hans P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1973
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC379898/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4357653/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/am.26.5.762-767.1973
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