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An in vitro assay for entry into cilia reveals unique properties of the soluble diffusion barrier

Specific proteins are concentrated within primary cilia, whereas others remain excluded. To understand the mechanistic basis of entry into cilia, we developed an in vitro assay using cells in which the plasma membrane was permeabilized, but the ciliary membrane was left intact. Using a diffusion-to-...

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Bibliographic Details
Main Authors: Breslow, David K., Koslover, Elena F., Seydel, Federica, Spakowitz, Andrew J., Nachury, Maxence V.
Format: Artigo
Language:Inglês
Published: The Rockefeller University Press 2013
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3798247/
https://ncbi.nlm.nih.gov/pubmed/24100294
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201212024
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