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“Super mutant” of yeast FMN adenylyltransferase enhances enzyme turnover rate by attenuating product inhibition
FMN adenylyltransferase (FMNAT) is an essential enzyme catalyzing the last step of a two-step pathway converting riboflavin (vitamin B2) to FAD, the ubiquitous flavocoenzyme. A structure-based mutagenesis and steady-state kinetic analysis of yeast FMNAT unexpectedly revealed that mutant D181A had a...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3795796/ https://ncbi.nlm.nih.gov/pubmed/23663086 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi400454w |
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