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Single-Molecule Analysis of Cytochrome c Folding by Monitoring the Lifetime of an Attached Fluorescent Probe

Conformational dynamics of proteins are important for function. However, obtaining information about specific conformations is difficult for samples displaying heterogeneity. Here, time-resolved fluorescence resonance energy transfer is used to characterize the folding of single cytochrome c molecul...

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Detalhes bibliográficos
Main Authors: Lee, Andrea J., Asher, Wesley B., Stern, Harry A., Bren, Kara L., Krauss, Todd D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3791595/
https://ncbi.nlm.nih.gov/pubmed/24116268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jz401259y
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