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Expression, Purification and Low-Resolution Structure of Human Vitamin C Transporter SVCT1 (SLC23A1)

Expression and purification of human membrane proteins for structural studies represent a great challenge. This is because micro- to milligram amounts of pure isolated protein are required. To this aim, we successfully expressed the human vitamin C transporter-1 (hSVCT1; SLC23A1) in Xenopus laevis o...

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Detalhes bibliográficos
Main Authors: Boggavarapu, Rajendra, Jeckelmann, Jean-Marc, Harder, Daniel, Schneider, Philipp, Ucurum, Zöhre, Hediger, Matthias, Fotiadis, Dimitrios
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3790709/
https://ncbi.nlm.nih.gov/pubmed/24124560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0076427
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