Developing a Novel Serum-Free Cell Culture Model of Skeletal Muscle Differentiation by Systematically Studying the Role of Different Growth Factors in Myotube Formation

This work describes the step-by-step development of a novel, serum-free, in vitro cell culture system resulting in the formation of robust, contracting, multinucleate myotubes from dissociated skeletal muscle cells obtained from the hind limbs of fetal rats. This defined system consisted of a serum-...

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Hlavní autoři: Das, Mainak, Rumsey, John W, Bhargava, Neelima, Gregory, Cassie, Reidel, Lisa, Kang, Jung Fong, Hickman, James J
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3787515/
https://ncbi.nlm.nih.gov/pubmed/19430851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11626-009-9192-7
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