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Rapid Conformational Epitope Mapping of anti-gp120 Antibodies with a Designed Mutant Panel Displayed on Yeast

gp120 is a substrate for protein engineering both for HIV immunogen design and as a bait for isolating anti-HIV antibodies from patient samples. In this work we describe the display of a stripped core gp120 on the yeast cell surface. Validation against a panel of neutralizing antibodies confirms tha...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Mata-Fink, Jordi, Kriegsman, Barry, Xin, Yu Hui, Zhu, Hanna, Hanson, Melissa, Irvine, Darrell J., Wittrup, K. Dane
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3785227/
https://ncbi.nlm.nih.gov/pubmed/23159556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.11.010
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