ProxiMAX randomization: a new technology for non-degenerate saturation mutagenesis of contiguous codons

Back in 2003, we published ‘MAX’ randomization, a process of non-degenerate saturation mutagenesis using exactly 20 codons (one for each amino acid) or else any required subset of those 20 codons. ‘MAX’ randomization saturates codons located in isolated positions within a protein, as might be requir...

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Autors principals: Ashraf, Mohammed, Frigotto, Laura, Smith, Matthew E., Patel, Seema, Hughes, Marcus D., Poole, Andrew J., Hebaishi, Husam R.M., Ullman, Christopher G., Hine, Anna V.
Format: Artigo
Idioma:Inglês
Publicat: Portland Press Ltd. 2013
Matèries:
Biochemical Society/Protein Society Focused Meeting
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3782830/
https://ncbi.nlm.nih.gov/pubmed/24059507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BST20130123
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