Improved genetically-encoded, FlincG-type fluorescent biosensors for neural cGMP imaging

Genetically-encoded biosensors are powerful tools for understanding cellular signal transduction mechanisms. In aiming to investigate cGMP signaling in neurones using the EGFP-based fluorescent biosensor, FlincG (fluorescent indicator for cGMP), we encountered weak or non-existent fluorescence after...

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Detalhes bibliográficos
Main Authors: Bhargava, Yogesh, Hampden-Smith, Kathryn, Chachlaki, Konstantina, Wood, Katherine C., Vernon, Jeffrey, Allerston, Charles K., Batchelor, Andrew M., Garthwaite, John
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2013
Assuntos:
Neuroscience
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3781335/
https://ncbi.nlm.nih.gov/pubmed/24068983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fnmol.2013.00026
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