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An Improved Targeted cAMP Sensor to Study the Regulation of Adenylyl Cyclase 8 by Ca(2+) Entry through Voltage-Gated Channels
Here we describe an improved sensor with reduced pH sensitivity tethered to adenylyl cyclase (AC) 8. The sensor was used to study cAMP dynamics in the AC8 microdomain of MIN6 cells, a pancreatic β-cell line. In these cells, AC8 was activated by Ca(2+) entry through L-type voltage-gated channels foll...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3781085/ https://ncbi.nlm.nih.gov/pubmed/24086669 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0075942 |
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