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Separation and Purification of Multiply Acetylated Proteins Using Cation-Exchange Chromatography

High-performance liquid chromatography (HPLC) is extremely useful for the study of proteins and the characterization of their posttranslational modifications. Here we describe a method that utilizes cation-exchange HPLC to separate multiply acetylated histone H3 species on the basis of their charge...

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Detalles Bibliográficos
Main Authors: Papazyan, Romeo, Taverna, Sean D.
Formato: Artigo
Idioma:Inglês
Publicado: 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3779653/
https://ncbi.nlm.nih.gov/pubmed/23381856
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-305-3_8
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