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Separation and Purification of Multiply Acetylated Proteins Using Cation-Exchange Chromatography
High-performance liquid chromatography (HPLC) is extremely useful for the study of proteins and the characterization of their posttranslational modifications. Here we describe a method that utilizes cation-exchange HPLC to separate multiply acetylated histone H3 species on the basis of their charge...
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Main Authors: | , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3779653/ https://ncbi.nlm.nih.gov/pubmed/23381856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-305-3_8 |
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