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Separation and Purification of Multiply Acetylated Proteins Using Cation-Exchange Chromatography
High-performance liquid chromatography (HPLC) is extremely useful for the study of proteins and the characterization of their posttranslational modifications. Here we describe a method that utilizes cation-exchange HPLC to separate multiply acetylated histone H3 species on the basis of their charge...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3779653/ https://ncbi.nlm.nih.gov/pubmed/23381856 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-305-3_8 |
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