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Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system

BACKGROUND: Linear DNA-based and Tol2-mediated transgenesis are powerful tools for the generation of transgenic zebrafish. However, the integration of multiple copies or transgenes at random genomic locations complicates comparative transgene analysis and makes long-term transgene stability unpredic...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Mosimann, Christian, Puller, Ann-Christin, Lawson, Katy L., Tschopp, Patrick, Amsterdam, Adam, Zon, Leonard I.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3775328/
https://ncbi.nlm.nih.gov/pubmed/23723152
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvdy.23989
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