Direct Cloning of Isogenic Murine DNA in Yeast and Relevance of Isogenicity for Targeting in Embryonic Stem Cells

Efficient gene targeting in embryonic stem cells requires that modifying DNA sequences are identical to those in the targeted chromosomal locus. Yet, there is a paucity of isogenic genomic clones for human cell lines and PCR amplification cannot be used in many mutation-sensitive applications. Here,...

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Detaylı Bibliyografya
Asıl Yazarlar: Andréasson, Claes, Schick, Anna J., Pfeiffer, Susanne M., Sarov, Mihail, Stewart, Francis, Wurst, Wolfgang, Schick, Joel A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2013
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3772885/
https://ncbi.nlm.nih.gov/pubmed/24058528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0074207
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