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Visualizing SNVs to quantify allele-specific expression in single cells

We present a high efficiency fluorescence in situ hybridization method for detecting single nucleotide variants (SNVs) on individual RNA transcripts, both exonic and intronic. We used this method to quantify allelic expression at the population and single cell level, and also to distinguish maternal...

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Bibliografski detalji
Glavni autori: Levesque, Marshall J., Ginart, Paul, Wei, Yichen, Raj, Arjun
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3771873/
https://ncbi.nlm.nih.gov/pubmed/23913259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2589
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