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Chromosome orientation fluorescence in situ hybridization (CO-FISH) to study sister chromatid segregation in vivo

Previously, assays for sister chromatid segregation patterns relied on incorporation of BrdU and indirect methods to infer segregation patterns after two cell divisions. Here we describe a method to differentially label sister chromatids of murine cells and directly assay sister chromatid segregatio...

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Bibliografiske detaljer
Main Authors: Falconer, Ester, Chavez, Elizabeth, Henderson, Alexander, Lansdorp, Peter M.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3771506/
https://ncbi.nlm.nih.gov/pubmed/20595964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.102
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