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Discrepancy between fluorescence correlation spectroscopy and fluorescence recovery after photobleaching diffusion measurements of G-protein-coupled receptors

Fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) are the two most direct methods to measure the diffusion of molecules in intact living cells. Ideally, these methods should produce similar results for an identical system. We have used these methods to...

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Detalhes bibliográficos
Main Authors: Calizo, Rhodora Cristina, Scarlata, Suzanne
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3770895/
https://ncbi.nlm.nih.gov/pubmed/23748145
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.04.033
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