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Discrepancy between fluorescence correlation spectroscopy and fluorescence recovery after photobleaching diffusion measurements of G-protein-coupled receptors

Fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy (FCS) are the two most direct methods to measure the diffusion of molecules in intact living cells. Ideally, these methods should produce similar results for an identical system. We have used these methods to...

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Autors principals: Calizo, Rhodora Cristina, Scarlata, Suzanne
Format: Artigo
Idioma:Inglês
Publicat: 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3770895/
https://ncbi.nlm.nih.gov/pubmed/23748145
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.04.033
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