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Agrobacterium-mediated genetic transformation using cotyledons in Japanese pear (Pyrus pyrifolia)

Genetic transformation was successfully established producing both transformed adventitious shoots and calli in Japanese pear (Pyrus pyrifolia Nakai) by using cotyledons as explants. Cotyledons of five cultivars were co-cultivated with Agrobacterium tumefaciens strain LBA4404 carrying the pBIN19-sgf...

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Detalhes bibliográficos
Main Authors: Nakajima, Ikuko, Sato, Yoshihiko, Saito, Toshihiro, Moriguchi, Takaya, Yamamoto, Toshiya
Formato: Artigo
Idioma:Inglês
Publicado em: Japanese Society of Breeding 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3770554/
https://ncbi.nlm.nih.gov/pubmed/24273422
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1270/jsbbs.63.275
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