A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study hi...

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Detalhes bibliográficos
Main Authors: Kumar, Gavish, Shankar, Hari, Bisht, Deepa, Sharma, Prashant, Singhal, Neelja, Katoch, V.M., Joshi, Beenu
Formato: Artigo
Idioma:Inglês
Publicado em: Sociedade Brasileira de Microbiologia 2010
Assuntos:
Medical Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3768669/
https://ncbi.nlm.nih.gov/pubmed/24031494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1517-83822010000200005
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