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Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simpli...

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Main Authors: von Groll, Andrea, Martin, Anandi, Portaels, Françoise, da Silva, Pedro Eduardo Almeida, Palomino, Juan Carlos
Format: Artigo
Jezik:Inglês
Izdano: Sociedade Brasileira de Microbiologia 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3768668/
https://ncbi.nlm.nih.gov/pubmed/24031495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1517-83822010000200006
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