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New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initia...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Sociedade Brasileira de Microbiologia
2009
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3768577/ https://ncbi.nlm.nih.gov/pubmed/24031424 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1517-83822009000400007 |
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