EMJH medium with 5-fluorouracil and nalidixic acid associated with serial dilution technique used to recover Leptospira spp from experimentally contaminated bovine semen

Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium with 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as “selective medium” and using the serial dilution technique, in order to evaluate the percentage of recov...

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Detalhes bibliográficos
Main Authors: Miraglia, Fabiana, de Moraes, Zenaide Maria, Melville, Priscilla Anne, Dias, Ricardo Augusto, Vasconcellos, Silvio Arruda
Formato: Artigo
Idioma:Inglês
Publicado em: Sociedade Brasileira de Microbiologia 2009
Assuntos:
Veterinary Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3768510/
https://ncbi.nlm.nih.gov/pubmed/24031342
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1517-838220090001000033
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